Monday, July 30, 2007

Laboratory Guidelines

General Guidelines

a. Conduct yourself in a responsible manner at all times in the laboratory.

b. Be familiar with your lab assignment before you come to lab. Follow all written and verbal instructions carefully. If you do not understand a direction or part of a procedure, ask the teacher before proceeding.

c. Never work alone. No student may work in the laboratory without an instructor present.

d. When first entering a science room, do not touch any equipment, chemicals, or other materials in the laboratory area until you are instructed to do so. \

e. Do not eat food, drink beverages, or chew gum in the laboratory. Do not use laboratory glassware as containers for food or beverages.

f. Perform only those experiments authorized by the instructor. Never do anything in the laboratory that is not called for in the laboratory procedures or by your instructor. Carefully follow all instructions, both written and oral. Unauthorized experiments are prohibited.

g. Observe good housekeeping practices. Work areas should be kept clean and tidy at all times. Bring only your laboratory instructions, worksheets, and/or reports to the work area. Other materials (books, purses, backpacks, etc.) should be stored in the classroom area.

h. Know the locations and operating procedures of all safety equipment including the first aid kit, eyewash station, safety shower, spill kit, fire extinguisher, and fire blanket. Know where the fire alarm and the exits are located.

i. Be alert and proceed with caution at all times in the laboratory. Notify the instructor immediately of any unsafe conditions you observe.

j. Labels and equipment instructions must be read carefully before use. Set up and use the prescribed apparatus as directed in the laboratory instructions provided by your teacher.

k. Keep hands away from your face, eyes, mouth, and body while using chemicals. Wash your hands with soap and water after performing all experiments. Clean (with detergent powder), rinse, and dry all work surfaces and equipment at the end of the experiment.

l. Experiments must be personally monitored at all times. You will be assigned a laboratory station at which to work. Do not wander around the room, distract other students, or interfere with the laboratory experiments of others.

m. If you spill acid or any other corrosive chemical on you skin or clothes immediately wash area with large amounts of water (remember that small amounts of water may be worse that no water at all). After this get the teacher’s attention. The spill kit will be used for spills on floor or counter-top.

n. At the end of the laboratory session see that: a) main gas outlet valve is shut off b) the water is turned off c) desk top, floor area, and sink are clean d) all equipment is cool, clean, and arranged.


a. Dress properly during a laboratory activity. Long hair, dangling jewelry, and loose or baggy clothing are a hazard in the laboratory. Long hair must be tied back and dangling jewelry and loose or baggy clothing must be secured. Shoes must completely cover the foot. No sandals are allowed.

Accidents and Injuries

a. Report any accident (spill, breakage, etc.) or injury (cut, burn, etc.) to the instructor immediately, no matter how trivial it may appear.

b. If a chemical should splash in your eye(s), immediately flush with running water from the eyewash station for at least 20 minutes. Notify the instructor immediately.

Handling Chemicals

a. All chemicals in the laboratory are to be considered dangerous. Do not touch, taste, or smell any chemical unless specifically instructed to do so. The proper technique for smelling chemical fumes (when instructed to do so by the teacher) is to gently fan the air above the chemical toward your face. Breathe normally.

b. Check the label on chemical bottles twice before removing any of the contents. Take only as much chemical as you need. Smaller amounts often work better than larger amounts. Label all containers and massing papers holding dry chemicals.

c. Never return unused chemicals to their original containers.

d. Never use mouth suction to fill a pipet. Use a pipet bulb or pipet filler.

e. Acids must be handled with extreme care. ALWAYS ADD ACID SLOWLY TO WATER, with slow stirring and swirling, being careful of the heat produced, particularly with sulfuric acid.

f. Handle flammable hazardous liquids over a pan to contain spills. Never dispense flammable liquids anywhere near an open flame or source of heat.

g. Never take chemicals or other materials from the laboratory area.

h. Take great care when transferring acids and other chemicals from one part of the laboratory to another. Hold them securely and in the method demonstrated by the teacher as you walk.

Heating Substances

a. Never leave a lit burner unattended. Never leave anything that is being heated or is visibly reacting unattended. Always turn the burner or hot plate off when not in use.

b. You will be instructed in the proper method of heating and boiling liquids in test tubes. Do not point the open end of a test tube being heated at yourself or anyone else.

c. Heated metals, glass, and ceramics remain very hot for a long time. They should be set aside to cool on a trivet and then picked up with caution. Use tongs or heat-protective gloves if necessary. Determine if an object is hot by bringing the back of your hand close to it prior to grasping it.


  • At 7:37 PM, Anonymous Cole-parmer said…

    The above points refers to laboratory guidelines. The guideline may also be used for the continuous education of laboratory personnel in order to train
    individuals in the art of specific IGE analysis as well as how to keep the processes and routines up to date. Thanks a lot...


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